Engineering topics

Vanalstine, J. M.

Publications and source records attributed to Vanalstine, J. M..

4 records

NASA NTRS1988

Hardware for studying the demixing of aqueous polymer two-phase systems in low gravity

The gravity-free demixing behavior of aqueous polymer two-phase systems (APTPS) has been studied on Earth, and onboard KC-135 aircraft and the Space Shuttle. Results suggest a good correlation between the demixing behavior of isopycnic systems on Earth and the low g behavior of systems exhibiting phase density differences. The short term (20 s) behavior of APTPS onboard KC-135 aircraft has been shown to mimic their initial demixing behavior in space. It appears that the few minutes of low g provided by parabolic rocket flight will be sufficient to study all but the later stages of demixing of APTPS. We are therefore turning our attention toward the design and use of hardware for studying the demixing of APTPS onboard sounding rockets. This seminar will discuss the evolution of hardware and research from hand-held KC-135 and Shuttle hardware through to automated hardware designed for utilization of KC-135, sounding rocket, and Space Shuttle Mid-deck Locker flight opportunities.

Vanalstine, J. M.↗

NASA NTRS1986

Separation of biological materials in microgravity

Partition in aqueous two phase polymer systems is a potentially useful procedure in downstream processing of both molecular and particulate biomaterials. The potential efficiency of the process for particle and cell isolations is much higher than the useful levels already achieved. Space provides a unique environment in which to test the hypothesis that convection and settling phenomena degrade the performance of the partition process. The initial space experiment in a series of tests of this hypothesis is described.

Brooks, D. E.↗

NASA NTRS1986

Demixing of aqueous polymer two-phase systems in low gravity

When polymers such as dextran and poly(ethylene glycol) are mixed in aqueous solution biphasic systems often form. On Earth the emulsion formed by mixing the phases rapidly demixes because of phase density differences. Biological materials can be purified by selective partitioning between the phases. In the case of cells and other particulates the efficiency of these separations appears to be somewhat compromised by the demixing process. To modify this process and to evaluate the potential of two-phase partitioning in space, experiments on the effects of gravity on phase emulsion demixing were undertaken. The behavior of phase systems with essentially identical phase densities was studied at one-g and during low-g parabolic aircraft maneuvers. The results indicate the demixing can occur rather rapidly in space, although more slowly than on Earth. The demixing process was examined from a theoretical standpoint by applying the theory of Ostwald ripening. This theory predicts demizing rates many orders of magnitude lower than observed. Other possible demixing mechanisms are considered.

Bamberger, S.↗

NASA NTRS1984

Separation of aqueous two-phase polymer systems in microgravity

Phase separation of polymer systems in microgravity is studied in aircraft flights to prepare shuttle experiments. Short duration (20 sec) experiments demonstrate that phase separation proceeds rapidly in low gravity despite appreciable phase viscosities and low liquid interfacial tensions (i.e., 50 cP, 10 micro N/m). Ostwald ripening does not appear to be a satisfactory model for the phase separation mechanism. Polymer coated surfaces are evaluated as a means to localize phases separated in low gravity. Contact angle measurements demonstrate that covalently coupling dextran or PEG to glass drastically alters the 1-g wall wetting behavior of the phases in dextran-PEG two phase systems.

Vanalstine, J. M.↗