Engineering topics
Publications and source records attributed to Wang, Taylor G..
An immunoisolation patch system, and particularly a patch system comprising multiple immunoisolation microcapsules, each encapsulating biological material such as cells for transplantation, which can be used in the prophylactic and therapeutic treatment of disease in large animals and humans without the need for immunosuppression.
The present invention provides a novel reactor for making capsules with uniform membrane. The reactor includes a source for providing a continuous flow of a first liquid through the reactor; a source for delivering a steady stream of drops of a second liquid to the entrance of the reactor; a main tube portion having at least one loop, and an exit opening, where the exit opening is at a height substantially equal to the entrance. In addition, a method for using the novel reactor is provided. This method involves providing a continuous stream of a first liquid; introducing uniformly-sized drops of the second liquid into the stream of the first liquid; allowing the drops to react in the stream for a pre-determined period of time; and collecting the capsules.
The present invention is drawn to a composition of matter comprising high viscosity sodium alginate, cellulose sulfate and a multi-component polycation. Additionally, the present invention provides methods for making capsules, measuring capsule permeability to immunologically-relevant proteins and treating disease in an animal using encapsulated cells. Over one thousand combinations of polyanions and polycations were examined as polymer candidates suitable for encapsulation of living cells and thirty-three pairs were effective. The combination of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine) hydrochloride, calcium chloride, and sodium chloride produced the most desirable results. Pancreatic islets encapsulated in this multicomponent capsule demonstrated glucose-stimulated insulin secretion in vitro and reversed diabetes without stimulating immune reaction in mice. The capsule formulation and system of the present invention allows independent adjustments of capsule size, wall thickness, mechanical strength and permeability, and offers distinct advantages for immunoisolating cells.
A method and apparatus for the accurate non-contact detection and measurement of static electric charge on an object using a reciprocating sensing probe that moves relative to the object. A monitor measures the signal generated as a result of this cyclical movement so as to detect the electrostatic charge on the object.
AA Using the existing inviscid theories, an attempt is made to explain the centering of the oscillating liquid shell. Experiments on liquid shells and liquid-core compound drops were conducted using acoustic levitation, in a low-gravity environment during a Space Shuttle flight. It was observed that their inner and outer interfaces became concentric when excited into capillary oscillations. Using the existing inviscid theories, and attempt is made to explain the centering of the oscillating liquid shell. It is concluded that viscosity needs to be considered in order to provide a realistic description of the centering process.
Experiments on rotational bifurcation of liquid drops, in which the drops were levitated and spun using acoustic fields in a low-gravity environment, were conducted during the first United States Microgravity Laboratory (USML-1) Space Shuttle flight. The experiments have successfully resolved the discrepancies existing between the previous experimental results and the theoretical predictions. In the case of a spherical drop, for which theory exists, the results agree well with the predictions. In the case of flattened drops, the experiments have extablished a family of curves, with the spherical drop as the limiting case.
The pressure dependence of the nucleation rate of an undercooled liquid is examined by analyzing the relationship between surface energy and external pressure. The dependence of the melt-crystal interfacial energy on the applied pressure is modeled, and the increase (decrease) in undercooling due to an applied pressure is described for substances which have positive (negative) values of delta-upsilon. A cavitation-nucleation argument is formulated along the lines of Hickling (1965) with attention given to the possibilities of negative and positive pulses about a bubble in the liquid.
A method for noncontact charge measurements is presented, which uses a system based on the reciprocal motion principle of a grounded conductor near a point charge. The system exhibits extremely good linearity with respect to the input electric field and has a resolution of greater than 10 V/m. The high sensitivity of the apparatus makes the system feasible for use at a safe distance in various applications for monitoring static charge.
The dynamics of mixing and satellite drop formation following the coalescence of initially stationary low-viscosity drops of the same liquid has been investigated using immiscible systems. The main parameters of interest are the drop sizes, the drop viscosity and the host viscosity. For drops of equal sizes there is no mixing following coalescence, which is consistent with that first demonstrated on the Skylab. However, for drops of unequal sizes there is considerable mixing with the smaller drop penetrating the larger drop as a vortex. In the extreme case of coalescence of a small drop with the bulk of the same liquid at a flat interface, the measured penetration depth is found to be proportional to the 5/4 th power of the drop diameter and inversely proportional to the square root of drop viscosity. When the drop and host viscosities are comparable, drop penetration is prematurely terminated by the formation of a satellite drop. However, when the drop diameter ratio (large to small) is below a critical number, satellite drop formation is inhibited and coalescence goes to completion. The influence of drop-host viscosity ratio on this critical diameter ratio has been examined.
The deformation and break-up are studied of a liquid drop in levitation through the radiation pressure. Using high-speed photography ripples are observed on the central membrane of the drop, atomization of the membrane by emission of satellite drops from its unstable ripples, and shattering of the drop after upward buckling like an umbrella, or after horizontal expansion like a sheet. These effects are captured on video. The ripples are theorized to be capillary waves generated by the Faraday instability excited by the sound vibration. Atomization occurs whenever the membrane becomes so thin that the vibration is sufficiently intense. The vibration leads to a destabilizing Bernoulli correction in the static pressure. Buckling occurs when an existent equilibrium is unstable to a radial (i.e., tangential) motion of the membrane because of the Bernoulli effect. Besides, the radiation stress at the rim of the drop is a suction stress which can make equilibrium impossible, leading to the horizontal expansion and the subsequent break-up.
A high efficiency acoustic chamber for the levitation and manipulation of liquid or molten samples in a microgravity environment has been developed. The chamber uses two acoustic drivers that are mounted at opposite corners of the chamber; by driving these at the same frequency, with 18-deg phase shifts, an increase in force of a factor of 3-4 is obtainable relative to the force of a single-driver system that is operated at the same power level. This enhancement is due to the increased coupling between the sound driver and the chamber. An anomalous rotation is noted to be associated with the chamber; this is found to be eliminated by a physically as-yet inexplicable empirical solution.
The hybrid, acoustic-EM levitator discussed provides a small lifting force independently of its EM component by exciting an acoustic resonance that serves as a pressure node at the position of the EM-levitated specimen. The system also stabilizes and damps chaotic oscillations during specimen positioning, and can excite forced oscillations of levitated molten metals for drop-physics and thermophysical property measurements. Attention is given to the character and function of the atmosphere in the levitator. Noncontact temperature measurement is via single-color optical pyrometer.
A new approach for detecting point type of charge has been developed. This system is based on the principle of reciprocal motion of a grounded conductor near a point charge. The current induced through such a process can then be used to determine the static field strength of the point charge, hence the quantity of the charge. Experiments were done to understand the characteristics of this new device. Test case indicates that the results are in good agreement with the theoretical prediction. This device shows extremely good linearity with respect to the strength of the input static field. The resolution of the system is better than 10 V/m, which is about two orders of magnitude in sensitivity higher than other types of field measuring apparatus. The high sensitivity of this system makes it possible to conduct charged drop experiments under microgravity conditions.
A new and systematic approach for producing polymer microspheres has been demonstrated. The membrane of the microsphere is formed by immersing the polyanionic droplet into a collapsing annular sheet, which is made of another polycation polymer solution. This method minimizes the impact force during the time when the chemical reaction takes place, hence eliminating the shortcomings of the current encapsulation techniques. The results of this study show the feasibility of this method for mass production of microcapsules.
A method is described for forming hollow particles, or shells, of extremely small size. The shell material is heated to a molten temperature in the presence of a gas that is at least moderately soluble in the shell material, to form a solution of the molten shell material and the soluble gas. The solution is atomized to form a multiplicity of separate droplets that are cooled while in free fall. Cooling of a droplet from the outside traps the desolved gas and forces it to form a gas bubble at the center of the droplet which now forms a gas filled shell. The shell is reheated and then cooled in free fall, in an environment having a lower pressure than the gas pressure in the shell. This causes expansion of the shell and the formation of a shell having a small wall thickness compared to its diameter.
Drop-dynamics experiments are described in terms of scope and methodology that are designed for the USML-1 flight. Included are experiments regarding: (1) the equilibrium shapes of acoustically rotated drops; (2) large-amplitude drop and bubble-shape oscillations; (3) the collision and coalescence of free drops; and (4) the deformation and instability of levitated drops. The space flight is concluded to provide an effective containerless microgravity environment for studying linear and nonlinear dynamics.
Hollow shells of high uniformity are formed by emitting liquid through an outer nozzle and gas through an inner nozzle, to form a hollow extrusion, by flowing the gas at a velocity between about 1.3 and 10 times the liquid velocity. The natural breakup rate of the extrusion can be increased to decrease shell size by applying periodic perturbations to one of the materials prior to exiting the nozzles, to a nozzle, or to the extrusion.
Simplifying assumptions lead to approximate closed-form solution. Theoretical paper discusses melting of solid sphere in spherical container. Develops mathematical model of melting process, based in part on simplifying assumptions like those used in theories of lubrication and film condensation. Resulting equation for melting speed as function of melting distance solved approximately in closed form.