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Willson, R. C.

Publications and source records attributed to Willson, R. C..

At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2001

Microbial detection with low molecular weight RNA

The need to monitor microorganisms in the environment has increased interest in assays based on hybridization probes that target nucleic acids (e.g., rRNA). We report the development of liquid-phase assays for specific bacterial 5S rRNA sequences or similarly sized artificial RNAs (aRNAs) using molecular beacon technology. These beacons fluoresce only in the presence of specific target sequences, rendering as much as a 27-fold fluorescence enhancement. The assays can be used with both crude cell lysates and purified total RNA preparations. Minimal sample preparation (e.g., heating to promote leakage from cells) is sufficient to detect many Gram-negative bacteria. Using this approach it was possible to detect an aRNA-labeled Escherichia coli strain in the presence of a large background of an otherwise identical E. coli strain. Finally, by using a longer wavelength carboxytetramethylrhodamine beacon it was possible to reduce the fraction of the signal due to cellular autofluorescence to below 0.5%.

NASA Discipline Life Sciences Technologies↗
NASA NTRS2001

RNA isolation and fractionation with compaction agents

A new approach to the isolation of RNA from bacterial lysates employs selective precipitation by compaction agents, such as hexammine cobalt and spermidine. Using 3.5 mM hexammine cobalt, total RNA can be selectively precipitated from a cell lysate. At a concentration of 2 mM hexammine cobalt, rRNA can be fractionated from low molecular weight RNA. The resulting RNA mixture is readily resolved to pure 5S and mixed 16S/23S rRNA by nondenaturing anion-exchange chromatography. Using a second stage of precipitation at 8 mM hexammine cobalt, the low molecular weight RNA fraction can be isolated by precipitation. Compaction precipitation was also applied to the purification of an artificial stable RNA derived from Escherichia coli 5S rRNA and to the isolation of an Escherichia coli-expressed ribozyme. Copyright 2001 Academic Press.

Non-NASA Center↗
NASA NTRS1999

Boronate affinity adsorption of RNA: possible role of conformational changes

Batch equilibrium adsorption isotherm determination is used to characterize the adsorption of mixed yeast RNA on agarose-immobilized m-aminophenylboronic acid. It is shown that the affinity-enhancing influence of divalent cations depends strongly on the precise nature of the cation used, with barium being far more effective than the conventionally-used magnesium. This adsorption-promoting influence of barium is suggested to arise primarily from ionic influences on the structure and rigidity of the RNA molecule, as the adsorption of ribose-based small molecules is not similarly affected. The substitution of barium for the standard magnesium counterion does not greatly promote the adsorption of DNA, implying that the effect is specific to RNA and may be useful in boronate-based RNA separations. RNA adsorption isotherms exhibit sharp transitions as functions of temperature, and these transitions occur at different temperatures with Mg2+ and with Ba2+. Adsorption affinity and capacity were found to increase markedly at lower temperatures, suggestive of an enthalpically favored interaction process. The stoichiometric displacement parameter, Z, in Ba2+ buffer is three times the value in Mg2+ buffer, and is close to unity.

NASA Discipline Environmental Health↗
NASA NTRS1995

Correlated brightness variations in solar radiative output from the photosphere to the corona

Correlated brightness variations are shown to occur in time series of coronal soft X-rays exclusive of prominent active regions, chromospheric ultraviolet radiation, and the photospheric total solar irradiance corrected for sunspot effects. These temporal correlations suggest that upwardly extending magnetic fields may have a large scale impact on the solar atmosphere in addition to their demonstrable role of generating localized active regions. The correlations have implications for improving and extending solar spectrum variability models.

Lean, J. L.↗
NASA NTRS1991

Variability of solar ultraviolet irradiance

A model of solar Lyman alpha irradiance developed by multiple linear regression analysis, including the daily values and 81-day running means of the full disk equivalent width of the Helium line at 1083 nm, predicts reasonably well both the short- and long-term variations observed in Lyman alpha. In contrast, Lyman alpha models calculated from the 10.7-cm radio flux overestimate the observed variations in the rising portion and maximum period of solar cycle, and underestimates them during solar minimum. Models are shown of Lyman alpha based on the He-line equivalent width and 10.7-cm radio flux for those time intervals when no satellite observations exist, namely back to 1974 and after April 1989, when the measurements of the Solar Mesosphere Satellite were terminated.

Pap, J. M.↗
NASA NTRS1991

Solar irradiance variability from modern measurements

Direct measurements from satellites of the solar 'constant' (the total irradiance at mean sun-earth distance) during more than ten years show variations over time scales from minutes to years and decades. At high frequencies, solar oscillations contribute to the variance. The most important influences are related to solar activity: during the passage of active regions on the solar disk (sunspots and faculae) changes of a few 0.1 percent lasting for several days are observed. The effects of spots can be well reproduced by the projected sunspot index, whereas the influence of faculae have to be modeled from proxy data like the Ca-K plage index or the He I index. Long-term trends are detected which are connected to the 11-yr solar activity cycle.

Froehlich, C.↗
NASA NTRS1990

Modeling Solar Lyman Alpha Irradiance

Solar Lyman alpha irradiance is estimated from various solar indices using linear regression analyses. Models developed with multiple linear regression analysis, including daily values and 81-day running means of solar indices, predict reasonably well both the short- and long-term variations observed in Lyman alpha. It is shown that the full disk equivalent width of the He line at 1083 nm offers the best proxy for Lyman alpha, and that the total irradiance corrected for sunspot effect also has a high correlation with Lyman alpha.

Pap, J.↗
NASA NTRS1988

Active Cavity Radiometer (ACR)

The objective of the Active Cavity Radiometer (ACR) experiment on the Atmospheric Laboratory for Applications and Science (ATLAS) mission is the measurement of the total solar irradiance with state-of-the-art accuracy and precision. This experiment is part of an ongoing program of space flight observations to study short- and long-term variations in the total solar output of optical energy. Precise observations of solar total irradiance provide information on the solar cycle and other long-term trends in solar output that are of climatological significance as well as short-term solar physics phenomena such as radiation anisotropy, active region structure, missing flux due to sunsports, bolometry of solar flares, global oscillations, coronal holes, and large-scale convective flows. The principal role of the ATLAS ACR observations will be in support of extended solar irradiance experiments on free-flying satellites. Annual in-flight comparison of observations by both ATLAS and free-flying experiments is an important part of sustaining the long-term precision of the climatological solar irradiance data base at the required + or - 0.1 percent level. Another role for ATLAS solar irradiance measurements will be establishment of the radiation scale at the solar total flux level in the International System of Units (SI). Two types of pyrheliometers, the ACR and SOLCON, will be directly intercompared during the ATLAS 1 mission. Addition of other sensors is planned for future reflights. Comparisons of solar observations by different pyrheliometers in the shuttle space environment will provide the most definitive experiment for determining their accuracy in defining the radiation scale at the solar total flux level.

Willson, R. C.↗
NASA NTRS1986

Long-term downward trend in total solar irradiance

The first 5 years (from 1980 to 1985) of total solar irradiance observations by the first Active Cavity Radiometer Irradiance Monitor (ACRIM I) experiment on board the Solar Maximum Mission spacecraft show a clearly defined downward trend of -0.019 percent/year. The existence of this trend has been confirmed by the internal self-calibrations of ACRIM I, by independent measurements from sounding rockets and balloons, and by observations from the Nimbus-7 spacecraft. The trend appears to be due to unpredicted variations of solar luminosity on time scales of years, and it may be related to solar cycle magnetic activity.

Willson, R. C.↗
NASA NTRS1984

Measurements of solar total irradiance and its variability

The development of electrically self calibrated cavity pyrheliometric instrumentation that occurred in the early 20th century provided the technological base for experiments to detect variability of the solar total irradiance. Experiments from ground based observatories, aircraft and balloons during the 1st half of the 20th century were unable to achieve sufficient accuracy or long term precision to unambiguously detect irradiance variations of solar origin. Refinements in pyrheliometric technology during the 1960's and 1970's and the accessibility of extended experimental opportunities above the earth's atmosphere in recent years have provided the first direct observations of solar total irradiance variability and provided the cornerstone observations of a long term database on solar irradiance. A program of solar irradiance monitoring has evolved to sustain the database over at least 22 years, corresponding to a single cycle of solar magnetic activity, and the shortest well identified cycle of climate variation. Direct links between total irradiance variations, solar magnetic activity and the solar global '5 min' oscillation phenomena have been derived from recent space flight observations by the SMM/ACRIM I experiment.

Willson, R. C.↗
NASA NTRS1984

The inconstant solar constant

The Active Cavity Radiometer Irradiance Monitor (ACRIM) of the Solar Maximum Mission satellite measures the radiant power emitted by the sun in the direction of the earth and has worked flawlessly since 1980. The main motivation for ACRIM's use to measure the solar constant is the determination of the extent to which this quantity's variations affect earth weather and climate. Data from the solar minimum of 1986-1987 is eagerly anticipated, with a view to the possible presence of a solar cycle variation in addition to that caused directly by sunspots.

Willson, R. C.↗
NASA NTRS1984

Solar Irradiance Variations on Active Region Time Scales

The variations of the total solar irradiance is an important tool for studying the Sun, thanks to the development of very precise sensors such as the ACRIM instrument on board the Solar Maximum Mission. The largest variations of the total irradiance occur on time scales of a few days are caused by solar active regions, especially sunspots. Efforts were made to describe the active region effects on total and spectral irradiance.

Labonte, B. J.↗
NASA NTRS1984

Solar Total Irradiance Variability Measurements by the SMM/ACRIM I Experiment

Convincing evidence of solar total irradiance variability and its relationships with solar activity was provided by the Active Cavity Radiometer Irradiance Monitor I (ACRIM I) experiment on the NASA Solar Maximum Mission (SMM). SMM/ACRIM I, the first flight experiment dedicated to the task of solar irradiance monitoring, has produced a multiyear solar total irradiance data base with + or - 0.02% or better long term precision since its launch in February, 1980. While the climatological significance of the results will not be apparent until many more years of continuous data are acquired, the discovery of variability on solar active region time scales has provided new insight into the physics of solar activity in the early years of the mission.

Willson, R. C.↗
NASA NTRS1983

Upper limits on the total radiant energy of solar flares

Limits on the total radiant energy of solar flares during the period February-November 1980 are established using data collected by the solar-constant monitor (ACRIM) on the Solar Maximum Mission satellite. Results show typical limits of 6 x 10 to the 29th erg/sec for a 32-second integration time, with 5-sigma statistical significance, for an impulsive emission. For a gradual component, about 4 x 10 to the 32nd ergs total radiant energy is found. The limits are determined to lie about an order of magnitude higher than the total radiant energy estimated from the various known emission components, which indicates the presence of a heretofore unknown dominant component of flare radiation.

Hudson, H. S.↗
NASA NTRS1982

Solar irradiance variations and solar activity

A mean value for the 1 AU total solar irradiance of 1368.2 W/sq m and a downward trend of 0.05% per year were derived from measurements by the Active Cavity Radiometer Irradiance Monitor (ACRIM) experiment on the Solar Maximum Mission during 1980. Distinct temporary solar irradiance decreases associated with solar activity maxima were observed with a series of nine dips from April to October recurring at fairly regular intervals averaging 24 days. The decreases correlate inversely with sunspot area, 2800-MHz flux, and Zurich sunspot number. Dominant periods common to the irradiance and sunspot area power spectra link the irradiance decreases to sunspot flux deficit in solar active regions. Evidence of significant total irradiance modulation by facular flux excess is cited. A persistent radiative cycle of active regions consistent with the ACRIM irradiance results and the morphology of solar active regions was found. The pattern of regularly recurrent active region maxima between April and October suggests an asymmetry in solar activity generation during this period.

Willson, R. C.↗
NASA NTRS1982

The effects of sunspots on solar irradiance

It is pointed out that the darkness of a sunspot on the visible hemisphere of the sun will reduce the solar irradiance on the earth. Approaches are discussed for obtaining a crude estimate of the irradiance deficit produced by sunspots and of the total luminosity reduction for the whole global population of sunspots. Attention is given to a photometric sunspot index, a global measure of spot flux deficit, and models for the compensating flux excess. A model is shown for extrapolating visible-hemisphere spot areas to the invisible hemisphere. As an illustration, this extrapolation is used to calculate a very simple model for the reradiation necessary to balance the flux deficit.

Hudson, H. S.↗