Probing DNA-protein interactions using single-molecule diffusivity contrast
Not Available
Wilson, Hugh↗
Engineering topics
Wilson, Hugh
Publications and source records attributed to Wilson, Hugh.
ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling
Not provided.
Biochemistry & Molecular Biology↗
ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling
Raw Data related to the manuscript: "ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling". Contains the following files: ReadMe.txt - a quick introduction to the upload; ABEL-FRET-data-format-exampleCode.docx - a detailed description of the data format, and description of two Example code files included in RawDataForZenodo.zip; RawDataForZenodo.zip - the raw data and example code files arranged in folders (one folder per experiment) and an additional folder for the example code files.
59 BASIC BIOLOGICAL SCIENCES↗
Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments
Significance Recent experiments have suggested that the energy released by a chemical reaction can propel its enzyme catalyst (for example, alkaline phosphatase). However, this topic remains controversial, partially due to the indirect and ensemble nature of existing measurements. Here, we used recently developed single-molecule approaches to monitor directly the motions of individual proteins in aqueous solution and find that single alkaline phosphatase enzymes do not diffuse faster under catalysis. Instead, we demonstrate that interactions between the fluorescent dye and the enzyme’s substrate can produce the signature of apparent diffusion enhancement in fluorescence correlation spectroscopy, the standard ensemble assay currently used to study enzyme diffusion and indicate that single-molecule approaches provide a more robust means to investigate diffusion at the nanoscale.
36 MATERIALS SCIENCE↗