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Engineering topics
Wong, C.
Publications and source records attributed to Wong, C..
Evidence of SV40 infections in hospitalized children
Simian virus 40 (SV40) is known to have contaminated poliovirus vaccines used between 1955 and 1963. Accumulating reports have described the presence of SV40 DNA in human tumors and normal tissues, although the significance of human infections by SV40 is unknown. We investigated whether unselected hospitalized children had evidence of SV40 infections and whether any clinical correlations were apparent. Serum samples were examined for SV40 neutralizing antibody using a specific plaque reduction test; of 337 samples tested, 20 (5.9%) had antibody to SV40. Seropositivity increased with age and was significantly associated with kidney transplants (6 of 15 [40%] positive, P < .001). Many of the antibody-positive patients had impaired immune systems. Molecular assays (polymerase chain reaction and DNA sequence analysis) on archival tissue specimens confirmed the presence of SV40 DNA in 4 of the antibody-positive patients. This study, using 2 independent assays, shows the presence of SV40 infections in children born after 1980. We conclude that SV40 causes natural infections in humans.
NASA Discipline Regulatory Physiology↗
Molecular evidence of simian virus 40 infections in children
Recent studies have detected simian virus 40 (SV40) DNA in certain human tumors and normal tissues. The significance of human infections by SV40, which was first discovered as a contaminant of poliovirus vaccines used between 1955 and 1963, remains unknown. The occurrence of SV40 infections in unselected hospitalized children was evaluated. Polymerase chain reaction and DNA sequence analyses were done on archival tissue specimens from patients positive for SV40 neutralizing antibody. SV40 DNA was identified in samples from 4 of 20 children (1 Wilms' tumor, 3 transplanted kidney samples). Sequence variation among SV40 regulatory regions ruled out laboratory contamination of specimens. This study shows the presence of SV40 infections in pediatric patients born after 1982.
Non-NASA Center↗
Global Validation of Single-Station Schumann Resonance Lightning Location
Global measurements of large, optically bright lightning events from the Optical Transient Detector (OTD) satellite are used to validate estimates of lightning location from single-station Schumann resonance (SR) data. Bearing estimates are obtained through conventional magnetic direction-finding techniques, while source range is estimated from the range-dependent impedance spectrum of individual SR transients. An analysis of 40 such transients suggests that single-station techniques can locate lightning globally with an accuracy of 1-2mm. This is confirmed by further validation at close ranges from flashes detected by the National Lightning Detection-Network (NLDN). Observations with both OTD and SR systems may be useful for globally locating lightning with necessary, if not sufficient, characteristics to trigger mesospheric sprites.
Boccippio, Dennis↗
Strip Mode SAR Processing at ASF
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hardware based processor high speed strip mode SAR↗
SAR data processing for the Magellan prime mission
The Magellan prime mission involves mapping the planet of Venus once around its rotational axis. The Magellan synthetic aperture radar (SAR) data processing facility processes all SAR data collected by the Magellan spacecraft into image data on an orbit-by-orbit basis. About 1700 million bits of radar data were collected. A complete description of the Magellan SAR Data Processing Facility is provided with emphasis on key design features of the data processors that satisfied the project data processing requirements. A summary of the attained data processing performance is included, as well as a brief discussion of some of the constraints and considerations regarding the applicability of the processors to meeting the data processing goals anticipated for the follow-on mission phases (i.e., cycles II, III, and beyond).
Leung, K.↗
Possible prebiotic significance of polyamines in the condensation, protection, encapsulation, and biological properties of DNA
Some properties of DNA condensed with spermidine have been compared with the properties of DNA condensed with Co3+(NH3)6 to determine whether condensation of DNA with these trivalent cations protects DNA against the action of DNase I and increases transcription and encapsulation of DNA into liposomes. It was shown that DNA condensed with Co3+(NH3)6 was resistant to the action of the endonuclease DNase I such as DNA condensed with spermidine was. However, DNA condensed with Co3+(NH3)6 was significantly less active in transcription with the E. coli RNA polymerase than DNA-spermidine condensed forms. In addition, it was demonstrated that both compacted forms of DNA were more efficiently encapsulated into neutral liposomes; however, negatively, charged liposomes were scarcely formed in the presence of DNA condensed with Co3+(NH3)6. These experiments and the well documented properties of polyamines increasing the resistance to radiations and hydrolysis of nucleic acids, as well as their biological activities, such as replication, transcription, and translation, together with the low concentration of Co3+ in the environment, lead us to propose spermidine as a plausible prebiotic DNA condensing agent rather than Co3+ and the basic proteins proposed by other authors. Then, we consider the possible role and relevance of the polyamine-nucleic acids complexes in the evolution of life.
NASA Discipline Exobiology↗
KBS V and V as related to automation of space station subsystems: Rationale for a KBS lifecycle
The role of verification and validation (V and V) in software has been to support and strengthen the software lifecycle and to ensure that the resultant code meets the standards of the requirements document. Knowledge based systems (KBS) V and V should serve the same role, but the KBS lifecycle is ill-defined. Here, the rationale of the simple form of the KBS lifecycle is explained. Special KBS development requirements are accommodated where possible by modifications to the traditional software lifecycle. Research areas are suggested for those aspects which present new or unusual difficulties for V and V.
Richardson, K.↗
Studies on precellular evolution - The encapsulation of polyribonucleotides by liposomes
Liposomes have been suggested as possible models of precellular systems formed in the early Archean earth from lipids of nonenzymatic origin. Since it is generally accepted that RNA molecules preceded double-stranded DNA molecules as genetic material, the encapsulation of polyribonucleotides within liposomes (made from dipalmitoyl phosphatidylcholine and from egg yolk phosphatidylcholine) was studied. Quantitative determinations show that approximately 50 percent of the available lipids form liposomes, and that up to 5 percent of the polyribonucleotides can be entrapped by them. Also studied was the encapsulation of polyribonucleotides in the presence of urea and cyanamide and of Zn(2+) and Pb(2+).
Baeza, I.↗
Appendage modal coordinate truncation criteria in hybrid coordinate dynamic analysis
The paper examines the validity of the assumption that certain appendage-distributed (modal) coordinates can be truncated from a system model without unacceptable degradation of fidelity in hybrid coordinate dynamic analysis for attitude control of spacecraft with flexible appendages. Alternative truncation criteria are proposed and their interrelationships defined. Particular attention is given to truncation criteria based on eigenvalues, eigenvectors, and controllability and observability. No definitive resolution of the problem is advanced, and exhaustive study is required to obtain ultimate truncation criteria.
Likins, P.↗