Engineering topics

Zhou, Julia

Publications and source records attributed to Zhou, Julia.

5 records

DOE OSTI2024

Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complex

Hachiman is a broad-spectrum antiphage defense system of unknown function. We show here that Hachiman is a heterodimeric nuclease-helicase complex, HamAB. HamA, previously a protein of unknown function, is the effector nuclease. HamB is the sensor helicase. HamB constrains HamA activity during surveillance of intact double-stranded DNA (dsDNA). When the HamAB complex detects DNA damage, HamB helicase activity activates HamA, unleashing nuclease activity. Hachiman activation degrades all DNA in the cell, creating "phantom" cells devoid of both phage and host DNA. We demonstrate Hachiman activation in the absence of phage by treatment with DNA-damaging agents, suggesting that Hachiman responds to aberrant DNA states. Phylogenetic similarities between the Hachiman helicase and enzymes from eukaryotes and archaea suggest deep functional symmetries with other important helicases across domains of life.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Genome expansion by a CRISPR trimmer-integrase

CRISPR–Cas adaptive immune systems capture DNA fragments from invading mobile genetic elements and integrate them into the host genome to provide a template for RNA-guided immunity. CRISPR systems maintain genome integrity and avoid autoimmunity by distinguishing between self and non-self, a process for which the CRISPR/Cas1–Cas2 integrase is necessary but not sufficient. In some microorganisms, the Cas4 endonuclease assists CRISPR adaptation, but many CRISPR–Cas systems lack Cas4. Here we show here that an elegant alternative pathway in a type I-E system uses an internal DnaQ-like exonuclease (DEDDh) to select and process DNA for integration using the protospacer adjacent motif (PAM). The natural Cas1–Cas2/exonuclease fusion (trimmer-integrase) catalyses coordinated DNA capture, trimming and integration. Five cryo-electron microscopy structures of the CRISPR trimmer-integrase, visualized both before and during DNA integration, show how asymmetric processing generates size-defined, PAM-containing substrates. Before genome integration, the PAM sequence is released by Cas1 and cleaved by the exonuclease, marking inserted DNA as self and preventing aberrant CRISPR targeting of the host. Together, these data support a model in which CRISPR systems lacking Cas4 use fused or recruited exonucleases for faithful acquisition of new CRISPR immune sequences.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2016

Optical Testing and Verification Methods for the James Webb Space Telescope Integrated Science Instrument Module Element

NASA's James Webb Space Telescope (JWST) is a 6.6m diameter, segmented, deployable telescope for cryogenic IR space astronomy (~40K). The JWST Observatory includes the Optical Telescope Element (OTE) and the Integrated Science Instrument Module (ISIM) that contains four science instruments (SI) and the fine guider. The SIs are mounted to a composite metering structure. The SI and guider units were integrated to the ISIM structure and optically tested at the NASA Goddard Space Flight Center as a suite using the Optical Telescope Element SIMulator (OSIM). OSIM is a full field, cryogenic JWST telescope simulator. SI performance, including alignment and wave front error, were evaluated using OSIM. We describe test and analysis methods for optical performance verification of the ISIM Element, with an emphasis on the processes used to plan and execute the test. The complexity of ISIM and OSIM drove us to develop a software tool for test planning that allows for configuration control of observations, associated scripts, and management of hardware and software limits and constraints, as well as tools for rapid data evaluation, and flexible re-planning in response to the unexpected. As examples of our test and analysis approach, we discuss how factors such as the ground test thermal environment are compensated in alignment. We describe how these innovative methods for test planning and execution and post-test analysis were instrumental in the verification program for the ISIM element, with enough information to allow the reader to consider these innovations and lessons learned in this successful effort in their future testing for other programs.

spaceborne telescopes↗

NASA NTRS2016

Cryo-Vacuum Testing of the JWST Integrated Science Instrument Module

In late 2015 early 2016, a major cryo-vacuum test was carried out for the Integrated Science Instrument Module (ISIM) of the James Webb Space Telescope. This test comprised the final cryo-certification and calibration test of the ISIM before its delivery for integration with the rest of the JWST observatory. Over the roughly 100-day period of the round-the-clock test program, the full complement of ISIM flight instruments, structure, harness radiator, and electronics were put through a comprehensive program of thermal, optical, electrical, and operational tests. We briefly summarize the goals, setup, execution, and key results for this critical JWST milestone.

cryo-vacuum test↗

NASA NTRS2016

Cryo-vacuum testing of the JWST Integrated Science Instrument Module (SPIE)

In late 2015/early 2016, a major cryo-vacuum test was carried out for the Integrated Science Instrument Module (ISIM) of the James Webb Space Telescope (JWST). This test comprised the final cryo-certification and calibration test of the ISIM, after its ambient environmental test program (vibration, acoustics, EMI/EMC), and before its delivery for integration with the rest of the JWST observatory. Over the 108-day period of the round-the-clock test program, the full complement of ISIM flight instruments, structure, harness radiator, and electronics were put through a comprehensive program of thermal, optical, electrical, and operational tests. The test verified the health and excellent performance of the instruments and ISIM systems, proving the ISIM element's readiness for integration with the telescope. We report here on the context, goals, setup, execution, and key results for this critical JWST milestone.

James Webb Space Telescope (JWST)↗