TY - RPRT TI - Conjugation-based genome engineering enables rapid prototyping and bioproduction in non-model bacteria AU - Guzman, Michael S. [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0000000238888055) AU - Kiattisewee, Cholpisit [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0000000263517352) AU - Comes, Jackson [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0009000167954162) AU - Robert, Amanda M. [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0009000862506636) AU - Cardiff, Ryan A.L. [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0009000105309010) AU - Cook, Margaret [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0000000342106885) AU - Scott, Allan [Biological Sciences Division, Pacific Northwest National Laboratory Richland, WA 99354, United States] (ORCID:0000000273857633) AU - Alba Burbano, Diego [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0000000284761506) AU - Anastasakis, Stella [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:000900009171396X) AU - Grube, Sarah [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0009000619198303) AU - Heiberg, Kieran [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States] (ORCID:0009000105581938) AU - Darst, Brian [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0009000848281880) AU - Howell, Daniel [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0000000292959774) AU - Dexter, Gara N. [Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN 37831, United States] (ORCID:0000000216905723) AU - Egbert, Robert G. [Biological Sciences Division, Pacific Northwest National Laboratory Richland, WA 99354, United States] (ORCID:0000000294707124) AU - Zalatan, Jesse G. [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States; Department of Chemistry, University of Washington, Seattle, WA 98195, United States] (ORCID:0000000214580654) AU - Guss, Adam M. [Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN 37831, United States] (ORCID:0000000158235329) AU - Elmore, Joshua R. [Biological Sciences Division, Pacific Northwest National Laboratory Richland, WA 99354, United States] (ORCID:0000000347509640) AU - Beliaev, Alexander S. [Biological Sciences Division, Pacific Northwest National Laboratory Richland, WA 99354, United States] (ORCID:0000000267664632) AU - Carothers, James M. [Department of Chemical Engineering, University of Washington, Seattle, WA 98195, United States; UW Center for Synthetic Biology and Molecular Engineering & Sciences Institute University of Washington, Seattle, WA 98195, United States] (ORCID:0000000167287833) PY - 2026 DO - 10.64898/2026.04.17.717921 UR - https://www.osti.gov/biblio/3364326 ID - 3364326 ER -