Task description for the 1983 catalog of tasks for the materials processing in space program

Abstract

Ground based research necessary to establish all of the optimum experimental conditions required to accomplish the best possible electrophoretic separation of human kidney cell fractions which produce urokinase, gravulocyte stimulating factor, or erythropoietin was carried out. This overall effort includes: (1) development of optimum buffer systems, (2) viability tests, (3) ground based research on electrophoretic mobilities, (4) development of standard cells, standard cell culture methods, and standard urokinase assay procedures, (5) acquisition of the ground control data to be compared with results using cells returned from the electrophoretic separations carried out in microgravity, and (6) ground based research on the electrophoretic mobilities of suspended pituitary cells.