Sample band spreading phenomena in ground and space-based electrophoretic separators

Abstract

Sample stream spreading in the transverse direction (direction perpendicular to the chamber center plane) is calculated for two ground-based and one space-based continuous flow electrophoresis chambers. The magnitude of transverse sample stream spreading is shown to be a function of the difference in electrical conductivity between the sample and curtain buffer. Sample/buffer conductivity mismatch distorts the normally uniform electrical field in the vicinity of the sample to produce transverse field components which, in turn, deflect sample material toward the chamber walls. These results indicate that nongravity effects are important and must be resolved in order for space-based continuous flow electrophoresis to achieve its maximum potential.