NASA NTRS · 19900034867
Cell separation in immunoaffinity partition in aqueous polymer two-phase systems
Abstract
Two methods for immunoaffinity partitioning are described. One technique involves the covalent coupling of poly (ethylene glycol) (PEG) to immunoglobulin G antibody preparations. In the second method PEG-modified Protein A is used to complex with cells and unmodified antibody. The effects of PEG molecular weight, the degree of modification, and varying phase system composition on antibody activity and its affinity for the upper phase are studied. It is observed that both methods resulted in effective cell separation.
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Karr, Laurel J., Van Alstine, James M., Snyder, Robert S., Shafer, Steven G., Harris, J. Milton. 1989-01-01. Cell separation in immunoaffinity partition in aqueous polymer two-phase systems. https://ntrs.nasa.gov/citations/19900034867
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