NASA NTRS · 19920024926
FNAS/advanced protein crystal growth
Abstract
A scintillation method is presented for determination of the temperature dependence of the solubility, S(T), of proteins in 50-100 micro-l volumes of solution. S(T) data for lysozyme and horse serum albumin were obtained for various combinations of pH and precipitant concentrations. The resulting kinetics and equilibrium information was used for dynamic control, that is the separation of nucleation and growth stages in protein crystallization. Individual lysozyme and horse serum albumin crystals were grown in 15-20 micro-l solution volumes contained in x-ray capillaries.
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Rosenberger, Franz. 1992-09-10. FNAS/advanced protein crystal growth. https://ntrs.nasa.gov/citations/19920024926
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