A comparison of imaging methods for use in an array biosensor

Golden, Joel P.; Ligler, Frances S.

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NASA NTRS · 20040088167

A comparison of imaging methods for use in an array biosensor

Golden, Joel P.·Ligler, Frances S.

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Abstract

An array biosensor has been developed which uses an actively-cooled, charge-coupled device (CCD) imager. In an effort to save money and space, a complementary metal-oxide semiconductor (CMOS) camera and photodiode were tested as replacements for the cooled CCD imager. Different concentrations of CY5 fluorescent dye in glycerol were imaged using the three different detection systems with the same imaging optics. Signal discrimination above noise was compared for each of the three systems.

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Evaluation Studies NASA Discipline Life Sciences Technologies Non-NASA Center Diagnostic Imaging/methods Video Recording/instrumentation/methods Microscopy, Fluorescence/instrumentation/methods Biosensing Techniques/instrumentation/methods Fluoroimmunoassay/instrumentation/methods Semiconductors Cold Reproducibility of Results Sensitivity and Specificity Equipment Design Comparative Study

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Golden, Joel P., Ligler, Frances S.. 2002-09-01. A comparison of imaging methods for use in an array biosensor. https://ntrs.nasa.gov/citations/20040088167

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