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NASA NTRS · 20050184298

Chromosome aberrations induced by high-LET radiations

Kawata, Tetsuya·Ito, Hisao·George, Kerry·Wu, Honglu·Cucinotta, Francis A.
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Abstract

Measurements of chromosome aberrations in peripheral blood lymphocytes are currently the most sensitive and reliable indicator of radiation exposure that can be used for biological dosimetry. This technique has been implemented recently to study radiation exposures incurred by astronauts during space flight, where a significant proportion of the dose is delivered by high-LET particle exposure. Traditional methods for the assessing of cytogenetic damage in mitotic cells collected at one time point after exposure may not be suitable for measuring high-LET radiation effects due to the drastic cell cycle perturbations and interphase cell death induced by this type of exposure. In this manuscript we review the recent advances in methodology used to study high-LET induced cytogenetic effects and evaluate the use of chemically-induced Premature Chromosome Condensation (PCC) as an alternative to metaphase analysis. Published data on the cytogenetic effects of in vitro exposures of high-LET radiation is reviewed, along with biodosimetry results from astronauts after short or long space missions.

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ISS ProjectFlight Experimentshort durationmannedlong durationSoyuz ProjectReview, TutorialReviewNASA Center JSCMir ProjectNASA Discipline Radiation HealthChromosome AberrationsSpace FlightCosmic RadiationMitosis/radiation effectsChromosomes/radiation effectsLymphocytes/radiation effectsInterphase/radiation effectsOxazolesFibroblasts/radiation effectsHumansResearch Support, U.S. Gov't, Non-P.H.SIn Situ Hybridization, FluorescenceEnzyme InhibitorsRelative Biological EffectivenessRadiation DosageLinear Energy TransferAstronautsPhosphoprotein Phosphatase/antagonists & inhibitorsWeightlessness

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Kawata, Tetsuya, Ito, Hisao, George, Kerry, Wu, Honglu, Cucinotta, Francis A.. 2004-12-01. Chromosome aberrations induced by high-LET radiations. https://ntrs.nasa.gov/citations/20050184298

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