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DOE OSTI · 1019570

Flow cytometric detection method for DNA samples

Nasarabadi, Shanavaz·Langlois, Richard G·Venkateswaran, Kodumudi S
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Abstract

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

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Nasarabadi, Shanavaz, Langlois, Richard G, Venkateswaran, Kodumudi S. 2011-07-05. Flow cytometric detection method for DNA samples. https://www.osti.gov/biblio/1019570

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