X-Ray Sources and High-Throughput Data Collection Methods

Snell, Gyorgy

doi:10.1007/978-1-61779-520-6_5

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DOE OSTI · 1034175

X-Ray Sources and High-Throughput Data Collection Methods

Snell, Gyorgy

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Abstract

X-ray diffraction experiments on protein crystals are at the core of the structure determination process. An overview of X-ray sources and data collection methods to support structure-based drug design (SBDD) efforts is presented in this chapter. First, methods of generating and manipulating X-rays for the purpose of protein crystallography, as well as the components of the diffraction experiment setup are discussed. SBDD requires the determination of numerous protein-ligand complex structures in a timely manner, and the second part of this chapter describes how to perform diffraction experiments efficiently on a large number of crystals, including crystal screening and data collection.

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59 BASIC BIOLOGICAL SCIENCES 60 APPLIED LIFE SCIENCES CRYSTALLOGRAPHY DATA PROCESSING DESIGN DIFFRACTION PROTEINS SYNCHROTRON RADIATION SYNCHROTRONS X-RAY DIFFRACTION X-RAY SOURCES

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Snell, Gyorgy. 2012-03-15. X-Ray Sources and High-Throughput Data Collection Methods. https://doi.org/10.1007/978-1-61779-520-6_5

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