Beyond the crystallization paradigm: Structure determination from diffraction patterns from ensembles of randomly oriented particles

Poon, H. C.; Saldin, D. K.

doi:10.1016/j.ultramic.2010.11.003

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DOE OSTI · 1060717

Beyond the crystallization paradigm: Structure determination from diffraction patterns from ensembles of randomly oriented particles

Poon, H. C.·Saldin, D. K.

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Abstract

We amplify on the priciples of the method we have recently proposed for recovering an oversampled diffraction pattern of a single particle from measured diffraction patterns from multiple particles in orientations related by rotation about an axis parallel to the incident radiation. We propose an alternative method of phasing a reference resolution ring by means of a non-negativity constraint on the diffraction intensities, point out the need for caution about enantiomeric ambiguities in the reconstruction of a diffraction pattern from its angular correlations, and show that converged correlations may be deduced by appropriate averaging of even very noisy data.

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59 BASIC BIOLOGICAL SCIENCES X-Ray Diffraction Disordered Ensembles of Identical Particles

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Poon, H. C., Saldin, D. K.. 2011-06-01. Beyond the crystallization paradigm: Structure determination from diffraction patterns from ensembles of randomly oriented particles. https://doi.org/10.1016/j.ultramic.2010.11.003

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