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DOE OSTI · 1384929

Highly confined surface imaging by solid immersion total internal reflection fluorescence microscopy

Wang, Lin·Vasilev, Cvetelin·Canniffe, Daniel P.·Wilson, Luke R.·Hunter, C. Neil·Cadby, Ashley J.
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solar (fuels), photosynthesis (natural and artificial), biofuels (including algae and biomass), bio-inspired, charge transport, membrane, synthesis (novel materials), synthesis (self-assembly)

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Wang, Lin, Vasilev, Cvetelin, Canniffe, Daniel P., Wilson, Luke R., Hunter, C. Neil, Cadby, Ashley J.. 2012-01-01. Highly confined surface imaging by solid immersion total internal reflection fluorescence microscopy. https://doi.org/10.1364/oe.20.003311

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Neutron and X-ray analysis of the Fenna–Matthews–Olson photosynthetic antenna complex from Prosthecochloris aestuarii

The Fenna–Matthews–Olson protein from Prosthecochloris aestuarii ( Pa FMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space group H 3, with unit-cell parameters a = b = 83.64, c = 294.78 Å, and diffracted X-rays to ∼1.7 Å resolution at room temperature. Large Pa FMO crystals grown to volumes of 0.3–0.5 mm 3 diffracted neutrons to 2.2 Å resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.

solar (fuels), photosynthesis (natural and artific↗