DOE OSTI · 1490639
Megahertz serial crystallography
Abstract
Here, the new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
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Wiedorn, Max O. (ORCID:0000000208562695), Oberthür, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian (ORCID:0000000165040503), Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Aplin, Steve, Awel, Salah, Ayyer, Kartik (ORCID:000000026881564X), Bajt, Saša, Barák, Imrich (ORCID:000000023690404X), Bari, Sadia (ORCID:0000000339852051), Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor (ORCID:0000000297004803), Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco (ORCID:0000000237655540), Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin (ORCID:000000034624023X), Dörner, Katerina, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias (ORCID:0000000252440791), Fromme, Petra, Gañán-Calvo, Alfonso M. (ORCID:0000000275526184), Gevorkov, Yaroslav (ORCID:0000000192737648), Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Göttlicher, Peter, Hajdu, Janos, Hauf, Steffen, Heymann, Michael (ORCID:0000000292788207), Holmes, Susannah, Horke, Daniel A. (ORCID:0000000298628108), Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoška, Juraj (ORCID:0000000172586256), Kobe, Bostjan (ORCID:0000000194139166), Kuhn, Manuela, Kupitz, Christopher, Küpper, Jochen (ORCID:0000000343959345), Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain (ORCID:0000000205695193), Xavier, P. Lourdu, Maia, Luis, Maia, Filipe R. N. C. (ORCID:000000022141438X), Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide (ORCID:0000000324396348), Michelat, Thomas (ORCID:0000000256898759), Mills, Grant, Monteiro, Diana C. F. (ORCID:0000000263187306), Morgan, Andrew (ORCID:0000000291634249), Mühlig, Kerstin, Munke, Anna (ORCID:0000000255102245), Münnich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Poehlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam, Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd (ORCID:0000000257780680), Schönherr, Robert, Schulz, Joachim, Sellberg, Jonas A. (ORCID:0000000327935052), Seibert, M. Marvin, Seuring, Carolin (ORCID:0000000310000859). 2018-10-02. Megahertz serial crystallography. https://doi.org/10.1038/s41467-018-06156-7
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