Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis

Thissen, James B.; Be, Nicholas A.; McLoughlin, Kevin; Gardner, Shea; Rack, Paul G.; Shapero, Michael H.; Rowland, Raymond R. R.; Slezak, Tom; Jaing, Crystal J.; Sawabe, Tomoo

doi:10.1371/journal.pone.0212045

DOE OSTI · 1512638

Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis

Thissen, James B.·Be, Nicholas A.·McLoughlin, Kevin·Gardner, Shea·Rack, Paul G.·Shapero, Michael H.·Rowland, Raymond R. R.·Slezak, Tom·Jaing, Crystal J.·Sawabe, Tomoo

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Abstract

Microarrays have proven to be useful in rapid detection of many viruses and bacteria. Pathogen detection microarrays have been utilized to diagnose viral and bacterial infections in clinical samples and to evaluate the safety of biological drug materials. In this study, the Axiom Microbiome Array was evaluated to discover its sensitivity, specificity and utility in microbiome analysis of veterinary clinical samples. The array contains probes designed to detect more than 12,000 species of viruses, bacteria, fungi, protozoa and archaea, yielding the most comprehensive microbial detection platform built to date. The array was able to detect Shigella and Aspergillus at 100 genome copies, and vaccinia virus DNA at 1,000 genome copies. The Axiom Microbiome Array made correct species-level calls in mock microbial community samples. When tested against serum, tissue, and fecal samples from pigs experimentally co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2, the microarray correctly detected these two viruses and other common viral and bacterial microbiome species. This cost-effective and high-throughput microarray is an efficient tool to rapidly analyze large numbers of clinical and environmental samples for the presence of multiple viral and bacterial pathogens.

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Thissen, James B., Be, Nicholas A., McLoughlin, Kevin, Gardner, Shea, Rack, Paul G., Shapero, Michael H., Rowland, Raymond R. R., Slezak, Tom, Jaing, Crystal J., Sawabe, Tomoo. 2019-02-08. Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis. https://doi.org/10.1371/journal.pone.0212045

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