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DOE OSTI · 1601815

A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps

Terwilliger, Thomas C.·Adams, Paul D.·Afonine, Pavel V.·Sobolev, Oleg V.
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Abstract

Here, we report a fully automated procedure for the optimization and interpretation of reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as phenix.map_to_model. We applied our approach to 476 datasets with resolution of 4.5 Å or better, including reconstructions of 47 ribosomes and 32 other protein–RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 Å or better and 47% for those at resolutions worse than 3 Å.

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BibTeXRIS

Terwilliger, Thomas C., Adams, Paul D., Afonine, Pavel V., Sobolev, Oleg V.. 2018-10-30. A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps. https://doi.org/10.1038/s41592-018-0173-1

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