Sequenceserver: A Modern Graphical User Interface for Custom BLAST Databases

Priyam, Anurag; Woodcroft, Ben J.; Rai, Vivek; Moghul, Ismail; Munagala, Alekhya; Ter, Filip; Chowdhary, Hiten; Pieniak, Iwo; Maynard, Lawrence J.; Gibbins, Mark Anthony; Moon, HongKee; Davis-Richardson, Austin; Uludag, Mahmut; Watson-Haigh, Nathan S.; Challis, Richard; Nakamura, Hiroyuki; Favreau, Emeline; Gómez, Esteban A.; Pluskal, Tomás; Leonard, Guy; Rumpf, Wolfgang; Wurm, Yannick

doi:10.1093/molbev/msz185

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Sequenceserver: A Modern Graphical User Interface for Custom BLAST Databases

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Abstract

Comparing newly obtained and previously known nucleotide and amino-acid sequences underpins modern biological research. BLAST is a well-established tool for such comparisons but is challenging to use on new data sets. We combined a user-centric design philosophy with sustainable software development approaches to create Sequenceserver, a tool for running BLAST and visually inspecting BLAST results for biological interpretation. Sequenceserver uses simple algorithms to prevent potential analysis errors and provides flexible text-based and visual outputs to support researcher productivity. Our software can be rapidly installed for use by individuals or on shared servers.

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59 BASIC BIOLOGICAL SCIENCES BLAST comparative genomics sequence analysis visualization

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Priyam, Anurag, Woodcroft, Ben J., Rai, Vivek, Moghul, Ismail, Munagala, Alekhya, Ter, Filip, Chowdhary, Hiten, Pieniak, Iwo, Maynard, Lawrence J., Gibbins, Mark Anthony, Moon, HongKee, Davis-Richardson, Austin, Uludag, Mahmut, Watson-Haigh, Nathan S., Challis, Richard, Nakamura, Hiroyuki, Favreau, Emeline, Gómez, Esteban A., Pluskal, Tomás, Leonard, Guy, Rumpf, Wolfgang, Wurm, Yannick. 2019-08-14. Sequenceserver: A Modern Graphical User Interface for Custom BLAST Databases. https://doi.org/10.1093/molbev%2Fmsz185

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