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DOE OSTI · 1624751

Axial Plane Optical Microscopy

Li, Tongcang·Ota, Sadao·Kim, Jeongmin·Wong, Zi Jing·Wang, Yuan·Yin, Xiaobo·Zhang, Xiang
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Abstract

We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample’s cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.

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Li, Tongcang, Ota, Sadao, Kim, Jeongmin, Wong, Zi Jing, Wang, Yuan, Yin, Xiaobo, Zhang, Xiang. 2014-12-01. Axial Plane Optical Microscopy. https://doi.org/10.1038/srep07253

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