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DOE OSTI · 1650870

Methods and apparatus for gnotobiotic plant growth

Kremer, James·Tiedje, James M.·He, Sheng-Yang
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Abstract

The disclosure relates to a plant growth apparatus and related system to grow and maintain plants under controlled biotic conditions, for example to grow axenic (microbe-free) plants, gnotobiotic (defined microbiota) plants, and holoxenic (complex, or undefined microbiota) plants. This system allows aseptic bottom irrigation with water, soluble nutrients, chemicals, and/or microbiota. Plants can be inverted for dipping and/or vacuum infiltration. The system also allows for passive (gravity) drainage, thereby allowing for gas exchange and preventing root anoxia. A variety of plant growth substrates can be used within the plant growth apparatus as a plant growth medium. The plant growth apparatus, containing the growth substrate medium, can be completely flushed via the drainage port to remove potential toxic byproducts of the sterilization processes. The entire system is suitably constructed using autoclavable material.

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59 BASIC BIOLOGICAL SCIENCES60 APPLIED LIFE SCIENCES

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BibTeXRIS

Kremer, James, Tiedje, James M., He, Sheng-Yang. 2020-05-12. Methods and apparatus for gnotobiotic plant growth. https://www.osti.gov/biblio/1650870

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