Engineering an increase in ethanol production by altering cofactor specificity

Lo, Jonathan; Guss, Adam M.; van Dijken, Johannes Pieter; Shaw, IV, Arthur J.; Olson, Daniel G.; Herring, Christopher D.; Argyros, D. Aaron; Caiazza, Nicky

DOE OSTI · 1735231

Engineering an increase in ethanol production by altering cofactor specificity

Lo, Jonathan·Guss, Adam M.·van Dijken, Johannes Pieter·Shaw, IV, Arthur J.·Olson, Daniel G.·Herring, Christopher D.·Argyros, D. Aaron·Caiazza, Nicky

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Abstract

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

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Lo, Jonathan, Guss, Adam M., van Dijken, Johannes Pieter, Shaw, IV, Arthur J., Olson, Daniel G., Herring, Christopher D., Argyros, D. Aaron, Caiazza, Nicky. 2020-09-08. Engineering an increase in ethanol production by altering cofactor specificity. https://www.osti.gov/biblio/1735231

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