DOE OSTI · 1742077
Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
Abstract
We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States.
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Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard, Burnham, ed., Carey-Ann D.. 2020-12-17. Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus. https://doi.org/10.1128/jcm.01764-20
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