Multiplexed binary assembly and quantitative tracking of bacterial populations

Gill, Ryan T.; Zeitoun, Ramsey Ibrahim

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DOE OSTI · 1771467

Multiplexed binary assembly and quantitative tracking of bacterial populations

Gill, Ryan T.·Zeitoun, Ramsey Ibrahim

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Abstract

Compositions and methods for tracking combinations of mutations in populations by coupling DNA assembly and paired-end sequencing technology with high-throughput sequencing. This allows for an unlimited number of mutation sites in a population of microorganisms to be identified, and allows sites to be sequenced across several replicates. Unique identifiers (DNA barcodes) can be used to measure fitness data by sequencing of the barcodes multiplexed with the mutation sites with high-throughput short sequencing read technology, allowing selections to be rapidly performed on populations of known combinatorial genotypes.

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Gill, Ryan T., Zeitoun, Ramsey Ibrahim. 2020-10-06. Multiplexed binary assembly and quantitative tracking of bacterial populations. https://www.osti.gov/biblio/1771467

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