DOE OSTI · 1781663
Counting mRNA copies in intact bacterial cells by fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH)
Abstract
A method for measuring mRNA copies in intact bacterial cells by fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) is presented. Unlike conventional single molecule FISH, where the presence of a transcript is determined by fluorescence intensity, fliFISH relies on On-Off duty cycles of photo-switching dyes to set a predetermined threshold for distinguishing true signals from background noise. The method provides a quantitative approach for detecting and counting true mRNA copies and rejecting false signals with high accuracy.
Keep this discovery
Explore connections, maps & timelines
Hu, Dehong, Cui, Yi, Markillie, Lye Meng, Chrisler, William B., Wang, Qian, Hatzenpichler, Roland S., Orr, Galya. 2021-02-13. Counting mRNA copies in intact bacterial cells by fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH). https://www.osti.gov/biblio/1781663
Cite the original work for its findings. Save a collection to share your selection of sources.