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DOE OSTI · 1820189

A gain series method for accurate EMCCD calibration

Ryan, Duncan P.·Dunlap, Megan K.·Gelfand, Martin P.·Werner, James H.·Van Orden, Alan K.·Goodwin, Peter M.
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Abstract

Abstract Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.

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36 MATERIALS SCIENCEEMCCDMaterial Sciencecalibrationcameraimaging and sensingmicroscopysingle-molecule biophysics

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Ryan, Duncan P., Dunlap, Megan K., Gelfand, Martin P., Werner, James H., Van Orden, Alan K., Goodwin, Peter M.. 2021-09-15. A gain series method for accurate EMCCD calibration. https://doi.org/10.1038/s41598-021-97759-6

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