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DOE OSTI · 1821239

MetaFunPrimer: an Environment-Specific, High-Throughput Primer Design Tool for Improved Quantification of Target Genes

Liu, Jia·Villanueva, Paul·Choi, Jinlyung·Gunturu, Santosh·Ouyang, Yang·Tiemann, Lisa K.·Cole, James R.·Glanville, Kathryn R.·Hall, Steven J.·McDaniel, Marshall D.·Lee, Jaejin·Howe, Adina·Bouskill, ed., Nick·Sanford, Robert (ORCID:0000000296079897)·Jul, Sul Woo
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Abstract

Amplification-based gene characterization allows for sensitive and specific quantification of functional genes. There is often a large diversity of genes represented for functional gene groups, and multiple primers may be necessary to target associated genes.

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59 BASIC BIOLOGICAL SCIENCESenvironment-specificfunctional gene quantificationnitrificationprimer designquantitative PCR

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Liu, Jia, Villanueva, Paul, Choi, Jinlyung, Gunturu, Santosh, Ouyang, Yang, Tiemann, Lisa K., Cole, James R., Glanville, Kathryn R., Hall, Steven J., McDaniel, Marshall D., Lee, Jaejin, Howe, Adina, Bouskill, ed., Nick, Sanford, Robert (ORCID:0000000296079897), Jul, Sul Woo. 2021-10-26. MetaFunPrimer: an Environment-Specific, High-Throughput Primer Design Tool for Improved Quantification of Target Genes. https://doi.org/10.1128/msystems.00201-21

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