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DOE OSTI · 1831488

Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Grupi, Asaf·Shapira, Zehavit·Yudovich, Shimon·Degani-Katzav, Nurit·Weiss, Shimon
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Abstract

Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)-based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA A antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

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Grupi, Asaf, Shapira, Zehavit, Yudovich, Shimon, Degani-Katzav, Nurit, Weiss, Shimon. 2021-07-24. Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs. https://doi.org/10.1016/j.bpr.2021.100007

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