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DOE OSTI · 1845662

ATAC-Seq Reveals an Isl1 Enhancer That Regulates Sinoatrial Node Development and Function

Galang, Giselle·Mandla, Ravi·Ruan, Hongmei·Jung, Catherine·Sinha, Tanvi·Stone, Nicole R.·Wu, Roland S.·Mannion, Brandon J.·Allu, Prasanna K. R.·Chang, Kevin·Rammohan, Ashwin·Shi, Marie B.·Pennacchio, Len A.·Black, Brian L.·Vedantham, Vasanth
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Abstract

Cardiac pacemaker cells (PCs) in the sinoatrial node (SAN) have a distinct gene expression program that allows them to fire automatically and initiate the heartbeat. Although critical SAN transcription factors, including Isl1 (Islet-1), Tbx3 (T-box transcription factor 3), and Shox2 (short-stature homeobox protein 2), have been identified, the cis-regulatory architecture that governs PC-specific gene expression is not understood, and discrete enhancers required for gene regulation in the SAN have not been identified. Here, to define the epigenetic profile of PCs using comparative ATAC-seq (assay for transposase-accessible chromatin with sequencing) and to identify novel enhancers involved in SAN gene regulation, development, and function.

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59 BASIC BIOLOGICAL SCIENCESArrhythmiasDevelopmental BiologyElectrophysiologyGene Expression and RegulationIsl1PhysiologySinoatrial nodecardiac conduction systemcis-regulatory elementgene regulationheart rate

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Galang, Giselle, Mandla, Ravi, Ruan, Hongmei, Jung, Catherine, Sinha, Tanvi, Stone, Nicole R., Wu, Roland S., Mannion, Brandon J., Allu, Prasanna K. R., Chang, Kevin, Rammohan, Ashwin, Shi, Marie B., Pennacchio, Len A., Black, Brian L., Vedantham, Vasanth. 2020-10-12. ATAC-Seq Reveals an Isl1 Enhancer That Regulates Sinoatrial Node Development and Function. https://doi.org/10.1161/circresaha.120.317145

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