Biases associated with a particular image volume metric

Abstract

Image analysis area and volume metrics A 80 and V 80 , used by others, are studied as a function of image morphology, a 3D feature, and Monte Carlo noise in simulated images. The A 80 area metric is found to be stable, with consistent results (and meaning) obtainable without a detailed understanding of 3D features and/or simulation noise levels. However, the V 80 volume metric varies significantly with image morphology, 3D features, and simulation noise. If an experimental image set gives A 80 area inferences that are consistent with 2D image simulations, but V 80 volume inferences that are significantly larger than those corresponding to simulated images, then issues related to 3D features and simulation noise should be explored before conclusions are drawn related to a possible mismatch in the real physical size of experimental and simulated source volumes.