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DOE OSTI · 1885708

Leveraging Super High Optical Resolution Microscopy to Probe the Interaction Zone Between Clostridium thermocellum and Biomass

Yarbrough, John·Stich, Dominik·Chung, Daehwan·Hengge, Neal·Vinzant, Todd·Huang, Stu·Zeng, Yining (ORCID:0000000202022479)·Donohoe, Bryon·Himmel, Michael·Bomble, Yannick·Tuskan, Gerald A.
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Abstract

This poster, part of the Pacific Northwest National Laboratory-sponsored Integration 2020: Visualizing the Proteome virtual conference, discusses super high optical resolution microscopy to probe the interaction zone between Clostritium thermocellum and biomass. Clostridium thermocellum is one of the most efficient microorganisms for the deconstruction of biomass. To achieve this high level of cellulolytic activity, C. thermocellum uses large multienzyme complexes known as cellulosomes to sugars in 4-5 break down polysaccharides found in plant cell walls. The attachment of bacterial switchgrass 70% cells to the nearby substrate via the cellulosome has been hypothesized to be the reason for this high efficiency. The region lying between the cell and the substrate has shown great variation and dynamics that are affected by the growth stage of cells and the substrate used for growth.

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bacteriabiomassBIOMASS FUELScellulosomeClostridium thermocellummicroscopysuper-high resolution

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Yarbrough, John, Stich, Dominik, Chung, Daehwan, Hengge, Neal, Vinzant, Todd, Huang, Stu, Zeng, Yining (ORCID:0000000202022479), Donohoe, Bryon, Himmel, Michael, Bomble, Yannick, Tuskan, Gerald A.. 2022-08-31. Leveraging Super High Optical Resolution Microscopy to Probe the Interaction Zone Between Clostridium thermocellum and Biomass. https://www.osti.gov/biblio/1885708

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