An improved process for the release of synthetic DNA sequences from a solid-phase capture support

Grajkowski, Andrzej; Cawrse, Brian M.; Takahashi, Mayumi; Beaucage, Serge L.

doi:10.1016/j.tetlet.2022.154077

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An improved process for the release of synthetic DNA sequences from a solid-phase capture support

Grajkowski, Andrzej·Cawrse, Brian M.·Takahashi, Mayumi·Beaucage, Serge L.

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Abstract

Here, to facilitate the solid-phase purification of synthetic DNA sequences, a riboside phosphoramidite, carrying a 5-O-capture linker and a 2-O-silyl ether protecting group, is incorporated into a DNA sequence during its last solid-phase synthesis cycle. After deprotection and release of the DNA sequence from the synthesis support, the sequence is then covalently linked to a capture support to enable the removal of shorter unbound DNA sequences by simply washing these off the support. The solid-phase purified DNA sequence is then released from the capture support, through an innovative intramolecular cyclodeesterification of its terminal riboside ethyl phosphate triester entity and is isolated in a yield of 94% while displaying an exquisite purity of 97%.

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59 BASIC BIOLOGICAL SCIENCES capture support ethyl phosphate triester intramolecular cyclodeesterification riboside phosphoramidite solid-phase purification solid-phase synthesis synthetic DNA sequences

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Grajkowski, Andrzej, Cawrse, Brian M., Takahashi, Mayumi, Beaucage, Serge L.. 2022-08-12. An improved process for the release of synthetic DNA sequences from a solid-phase capture support. https://doi.org/10.1016/j.tetlet.2022.154077

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