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DOE OSTI · 1984695

Developing High-Flux Ion Soft Landing with Mass-Selection for Improved Cryo-Electron Microscopy

Powell, Samantha M.·Zhou, Mowei·Evans, James E.·Johnson, Grant E.·Paša-Tolić, Ljiljana
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Abstract

The goal of this project is to redesign and repurpose existing high-flux ion soft-landing (SL) instrumentation developed at PNNL for new structural biology applications by integrating SL, mass-selection/separation of high mass-to-charge species, native mass spectrometry (MS), and cryo-electron microscopy (EM) to generate a workflow enabling structural and functional annotation of proteins and other complex biological samples. Ultimately, this work will result in a new instrument for mass-selection and SL based cryo-EM sample preparation and potentially increase the impact of PNNL and the broader microscopy community through commercial licensing.

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BibTeXRIS

Powell, Samantha M., Zhou, Mowei, Evans, James E., Johnson, Grant E., Paša-Tolić, Ljiljana. 2022-09-01. Developing High-Flux Ion Soft Landing with Mass-Selection for Improved Cryo-Electron Microscopy. https://doi.org/10.2172/1984695

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