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DOE OSTI · 2228971

NIR fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera

Michalet, Xavier·Ulku, Arin C.·Wayne, Michael A.·Weiss, Shimon·Bruschini, Claudio·Charbon, Edoardo
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Abstract

SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (width W < 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. Furthermore, the results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.

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47 OTHER INSTRUMENTATIONNIRSPADdyesfluorescencefluorescence lifetime imaginggated imaginglifetimenear infraredphasorpulsed laser operationsingle photon avalanche diodestime-gatedwidefield

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Michalet, Xavier, Ulku, Arin C., Wayne, Michael A., Weiss, Shimon, Bruschini, Claudio, Charbon, Edoardo. 2023-04-25. NIR fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera. https://doi.org/10.1117/12.2649227

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