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DOE OSTI · 23082484

Glucocorticoids indirectly decrease colon cancer cell proliferation and invasion via effects on cancer-associated fibroblasts

Drebert, Zuzanna·De Vlieghere, Elly·Bridelance, Jolien·De Wever, Olivier·De Bosscher, Karolien·Bracke, Marc
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Abstract

Highlights: • Indirect effects of glucocorticoid treatment on cancer cells via CAFs are described. • Glucocorticoids decrease expression of MMP-2 by CAFs. • Glucocorticoids neutralize growth-promoting and pro-invasive effects of CAFs. • In vivo, glucocorticoid treatment inhibits tumor progression. Cancer-associated fibroblasts (CAFs) support cancer growth, invasion, and metastasis. Glucocorticoids (GCs), drugs often administered together with chemotherapy, are steroidal ligands of the glucocorticoid receptor (GR), a transcription factor which upon activation regulates expression of multiple genes involved in suppression of inflammation. We have previously shown that in dexamethasone (Dex)-treated CAFs derived from colon cancer, production and secretion of several factors related to cancer progression, such as tenascin C (TNC) and hepatocyte growth factor (HGF), were strongly suppressed. In this study we show that GCs can neutralize the cancer cell-promoting properties of CAFs. Conditioned medium from solvent-treated CAFs (CM{sup CTRL}) stimulates proliferation, motility and stretched morphotype of GR-deficient HCT8/E11 colon cancer cells. Yet, HCT8/E11 proliferation and stretched morphotype are impaired upon treatment with conditioned medium from Dex-treated CAFs (CM{sup DEX}), but HCT8/E11 cell migration is slightly increased under these conditions. Moreover, expression and potential activity of MMP-2 is also reduced in CM{sup DEX} compared with CM{sup CTRL}. These combined in vitro results concur with the results from in vivo chick chorioallantoic membrane assays, where the co-cultures of CAFs with colon cancer cells displayed impaired tumor formation and cancer cell invasion due to Dex administration. Combined, GC treatment influences cancer cell behavior indirectly through effects on CAFs.

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Drebert, Zuzanna, De Vlieghere, Elly, Bridelance, Jolien, De Wever, Olivier, De Bosscher, Karolien, Bracke, Marc. 2018-01-15. Glucocorticoids indirectly decrease colon cancer cell proliferation and invasion via effects on cancer-associated fibroblasts. https://doi.org/10.1016/j.yexcr.2017.11.034

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