Trichoderma harzianum transcriptome in response to the nematode Pratylenchus brachyurus

de Oliveira, Camilla Martins; Oshiquiri, Letícia Harumi; Almeida, Nayane Oliveira; Steindorf, Andrei Stecca; da Rocha, Mara Rúbia; Georg, Raphaela Castro; Ulhoa, Cirano José

doi:10.1016/j.biocontrol.2023.105245

DOE OSTI · 2422798

Trichoderma harzianum transcriptome in response to the nematode Pratylenchus brachyurus

de Oliveira, Camilla Martins·Oshiquiri, Letícia Harumi·Almeida, Nayane Oliveira·Steindorf, Andrei Stecca·da Rocha, Mara Rúbia·Georg, Raphaela Castro·Ulhoa, Cirano José

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Abstract

The root-lesion nematode Pratylenchus brachyurus causes extensive damage in several crops of economic importance. Fungi of Trichoderma genus have been highlighted as biopesticide agents in the control of several plant diseases. Although it is already widely used in agriculture, there are few studies, especially at the molecular level, that evaluate T. harzianum in the control of P. brachyurus . The aim of the present study was to investigate how interaction with the nematode P. brachyurus influences gene expression of T. harzianum , by using RNA-Seq analysis. Of the 13,932 predicted genes in the T. harzianum genome, 2,922 (21%) were differentially expressed in the presence of P. brachyurus , in relation to the absence of the nematode. Among the differentially expressed genes, we found genes encoding Carbohydrate Active EnZymes (CAZy), MEROPS peptidases, and proteins related to secondary metabolite synthesis. 118 pathways were identified as related to the biosynthesis of secondary metabolites. Additionally, the analysis identified 136 metabolic pathways related to these differentially expressed genes, among which we highlight: aminobenzoate degradation, xenobiotic metabolism by cytochrome P450, and sesquiterpenoid and triterpenoid biosynthesis. Our results contribute to a better understanding of the response of T. harzianum to the nematode P. bracyurus , the potential for biocontrol by this fungus.

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59 BASIC BIOLOGICAL SCIENCES Biological control Biotechnology & Applied Microbiology Entomology Gene expression Root-lesion nematode Secondary metabolites Trichoderma spp.

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de Oliveira, Camilla Martins, Oshiquiri, Letícia Harumi, Almeida, Nayane Oliveira, Steindorf, Andrei Stecca, da Rocha, Mara Rúbia, Georg, Raphaela Castro, Ulhoa, Cirano José. 2023-05-02. Trichoderma harzianum transcriptome in response to the nematode Pratylenchus brachyurus. https://doi.org/10.1016/j.biocontrol.2023.105245

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