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DOE OSTI · 2471468

The PagKNAT2/6b-PagBOP1/2a Regulatory Module Controls Leaf Morphogenesis in Populus

Zhao, Yanqiu·Zhang, Yifan·Zhang, Weilin·Shi, Yangxin·Jiang, Cheng·Song, Xueqin·Tuskan, Gerald A.·Zeng, Wei·Zhang, Jin·Lu, Mengzhu
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Abstract

Leaf morphogenesis requires precise regulation of gene expression to achieve organ separation and flat-leaf form. The poplar KNOTTED-like homeobox gene PagKNAT2/6b could change plant architecture, especially leaf shape, in response to drought stress. However, its regulatory mechanism in leaf development remains unclear. In this work, gene expression analyses of PagKNAT2/6b suggested that PagKNAT2/6b was highly expressed during leaf development. Moreover, the leaf shape changes along the adaxial-abaxial, medial-lateral, and proximal-distal axes caused by the mis-expression of PagKNAT2/6b demonstrated that its overexpression (PagKNAT2/6b OE) and SRDX dominant repression (PagKNAT2/6b SRDX) poplars had an impact on the leaf axial development. The crinkle leaf of PagKNAT2/6b OE was consistent with the differential expression gene PagBOP1/2a (BLADE-ON-PETIOLE), which was the critical gene for regulating leaf development. Further study showed that PagBOP1/2a was directly activated by PagKNAT2/6b through a novel cis-acting element “CTCTT”. Together, the PagKNAT2/6b-PagBOP1/2a module regulates poplar leaf morphology by affecting axial development, which provides insights aimed at leaf shape modification for further improving the drought tolerance of woody plants.

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59 BASIC BIOLOGICAL SCIENCESBiochemistry & Molecular BiologyChemistryPagBOP1/2aPagKNAT2/6bPopulusleaf morphogenesis

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Zhao, Yanqiu, Zhang, Yifan, Zhang, Weilin, Shi, Yangxin, Jiang, Cheng, Song, Xueqin, Tuskan, Gerald A., Zeng, Wei, Zhang, Jin, Lu, Mengzhu. 2022-05-17. The PagKNAT2/6b-PagBOP1/2a Regulatory Module Controls Leaf Morphogenesis in Populus. https://doi.org/10.3390/ijms23105581

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