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DOE OSTI · 2472185

Cofactor maturase NifEN: A prototype ancient nitrogenase?

Lee, Chi Chung·Górecki, Kamil·Stang, Martin·Ribbe, Markus W.·Hu, Yilin
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Abstract

Nitrogenase plays a key role in the global nitrogen cycle; yet, the evolutionary history of nitrogenase and, particularly, the sequence of appearance between the homologous, yet distinct NifDK (the catalytic component) and NifEN (the cofactor maturase) of the extant molybdenum nitrogenase, remains elusive. Here, we report the ability of NifEN to reduce N 2 at its surface-exposed L-cluster ([Fe 8 S 9 C]), a structural/functional homolog of the M-cluster (or cofactor; [(R-homocitrate)MoFe 7 S 9 C]) of NifDK. Furthermore, we demonstrate the ability of the L-cluster–bound NifDK to mimic its NifEN counterpart and enable N 2 reduction. These observations, coupled with phylogenetic, ecological, and mechanistic considerations, lead to the proposal of a NifEN-like, L-cluster–carrying protein as an ancient nitrogenase, the exploration of which could shed crucial light on the evolutionary origin of nitrogenase and related enzymes.

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Lee, Chi Chung, Górecki, Kamil, Stang, Martin, Ribbe, Markus W., Hu, Yilin. 2024-06-12. Cofactor maturase NifEN: A prototype ancient nitrogenase?. https://doi.org/10.1126/sciadv.ado6169

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