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DOE OSTI · 2510500

PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Transcriptomics (PB-DP3)

Anderson, Lindsey N [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); PNNL; Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]·Johnson, Zachary D [Washington State University Tri-Cities, Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]·Waters, Katrina M [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]·Bohutskyi, Pavlo [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]
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Abstract

The purpose of this experiment was to evaluate how circadian clock regulation impacts carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 in providing insights to strategies for enhanced bioproduction. Sample data was acquired using a Illumina HiSeq sequencer system and processed for RNA sequencing (RNA-Seq) expression analysis. Transcriptomic differential expression analysis revealed coordinated circadian clock-driven adjustment of the cell cycle and rewiring of energy and carbon metabolism. Processed RNA-Seq datasets are openly accessible from the PNNL DataHub project dataset download page and contain secondary processed RNA-seq results files and supporting metadata materials linked to relevant source code information supporting data transparency and reuse.

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59 BASIC BIOLOGICAL SCIENCESCarbohydrate MetabolismCarbon CyclingCircadian RegulationPredictive PhenomicsRedox BalancingRNA-SeqS. elongatus PCC 7942

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Anderson, Lindsey N [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); PNNL; Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Johnson, Zachary D [Washington State University Tri-Cities, Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Waters, Katrina M [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Bohutskyi, Pavlo [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]. 2024-12-30. PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Transcriptomics (PB-DP3). https://doi.org/10.25584/2510500

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