Reversible enzyme-catalysed NAD + /NADH electrochemistry

Giang, Peter D. [School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia] (ORCID:000000021408997X); Niks, Dimitri [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000176094996); Hakopian, Sheron [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000229179767); Hille, Russ [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000218918958); Bernhardt, Paul V. [School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia] (ORCID:0000000168391763)

doi:10.1039/d5sc00570a

DOE OSTI · 2529493

Reversible enzyme-catalysed NAD + /NADH electrochemistry

Giang, Peter D. [School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia] (ORCID:000000021408997X)·Niks, Dimitri [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000176094996)·Hakopian, Sheron [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000229179767)·Hille, Russ [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000218918958)·Bernhardt, Paul V. [School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia] (ORCID:0000000168391763)

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Abstract

The formate dehydrogenase subcomplex FdsBG from Cupriavidus necator has been electrochemically activated to reversibly interconvert NAD + /NADH and provide unique insight to its kinetics and reactivity.

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Giang, Peter D. [School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia] (ORCID:000000021408997X), Niks, Dimitri [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000176094996), Hakopian, Sheron [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000229179767), Hille, Russ [Department of Biochemistry, University of California, Riverside, USA] (ORCID:0000000218918958), Bernhardt, Paul V. [School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia] (ORCID:0000000168391763). 2025-04-02. Reversible enzyme-catalysed NAD + /NADH electrochemistry. https://doi.org/10.1039/d5sc00570a

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