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DOE OSTI · 2564112

Adding labile carbon to peatland soils triggers deep carbon breakdown

Rajakaruna, Sumudu [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0000000318363726)·Makke, Ghiwa [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0009000430308927)·Grachet, Nathalia Graf [Univ. of Arizona, Tucson, AZ (United States)]·Ayala-Ortiz, Christian [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0000000181542378)·Bouranis, John [Univ. of Arizona, Tucson, AZ (United States)]·Hoyt, David W. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:000000022857719X)·Toyoda, Jason G. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000162992146)·Denis, Elizabeth H. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000241505043)·Moran, James J. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Michigan State Univ., East Lansing, MI (United States)] (ORCID:0000000190819017)·Song, Tianze [Georgia Institute of Technology, Atlanta, GA (United States)] (ORCID:0000000282060247)·Sun, Xiaoxu [Georgia Institute of Technology, Atlanta, GA (United States)]·Eder, Elizabeth K. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]·Wong, Allison R. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]·Chu, Rosalie [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000174287647)·Heyman, Heino [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Metabolon, Inc., Durham, NC (United States)] (ORCID:0000000309412506)·Kolton, Max [Ben Gurion Univ. of the Negev, Beer Sheva (Israel)] (ORCID:0000000281663572)·Chanton, Jeffrey P. [Florida State Univ., Tallahassee, FL (United States)] (ORCID:0000000233039708)·Wilson, Rachel M. [Florida State Univ., Tallahassee, FL (United States)]·Kostka, Joel [Georgia Institute of Technology, Atlanta, GA (United States)] (ORCID:0000000330511330)·Tfaily, Malak M. [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0000000230362833)
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Abstract

Peatlands store vast amounts of carbon, with deep peat carbon remaining stable due to limited thermodynamic energy and transport. However, climate change-induced increases in labile carbon inputs could destabilize these stores. Here, we combined DNA stable isotope probing with stable isotope-assisted metabolomics employing a multi-platform approach to investigate microbial dynamics driving deep peat carbon degradation upon labile carbon (e.g., glucose) amendment. Our findings highlight the vulnerability of deep peat carbon, as glucose addition triggers the breakdown of older organic matter. By uniquely integrating these techniques, we identified active glucose metabolizers to specific microbial populations and mapped carbon flow through microbial networks, elucidating their role in priming recalcitrant carbon mineralization. This multi-omics approach offers crucial insights into how changing resources reshape the peatland microbiome, enhancing our understanding of deep carbon processing, and refining model parameterization to predict microbial responses and carbon cycle feedbacks under global change pressures.

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59 BASIC BIOLOGICAL SCIENCESDNA-SIPSIP-metabolomicscarbon cyclemicrobial ecologypeatlandprimingsoil microbiota

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Rajakaruna, Sumudu [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0000000318363726), Makke, Ghiwa [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0009000430308927), Grachet, Nathalia Graf [Univ. of Arizona, Tucson, AZ (United States)], Ayala-Ortiz, Christian [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0000000181542378), Bouranis, John [Univ. of Arizona, Tucson, AZ (United States)], Hoyt, David W. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:000000022857719X), Toyoda, Jason G. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000162992146), Denis, Elizabeth H. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000241505043), Moran, James J. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Michigan State Univ., East Lansing, MI (United States)] (ORCID:0000000190819017), Song, Tianze [Georgia Institute of Technology, Atlanta, GA (United States)] (ORCID:0000000282060247), Sun, Xiaoxu [Georgia Institute of Technology, Atlanta, GA (United States)], Eder, Elizabeth K. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Wong, Allison R. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Chu, Rosalie [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000174287647), Heyman, Heino [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Metabolon, Inc., Durham, NC (United States)] (ORCID:0000000309412506), Kolton, Max [Ben Gurion Univ. of the Negev, Beer Sheva (Israel)] (ORCID:0000000281663572), Chanton, Jeffrey P. [Florida State Univ., Tallahassee, FL (United States)] (ORCID:0000000233039708), Wilson, Rachel M. [Florida State Univ., Tallahassee, FL (United States)], Kostka, Joel [Georgia Institute of Technology, Atlanta, GA (United States)] (ORCID:0000000330511330), Tfaily, Malak M. [Univ. of Arizona, Tucson, AZ (United States)] (ORCID:0000000230362833). 2024-12-27. Adding labile carbon to peatland soils triggers deep carbon breakdown. https://doi.org/10.1038/s43247-024-01954-y

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