DOE OSTI · 2587192
Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
Abstract
This workflow enables lamella production targeting fluorescently labeled biological structures that are small (<1 μm in axial extent) and rare (1 copy per cell) using a cryogenic tri-coincident imaging platform. In conclusion, this platform integrates fluorescence microscopy, focused ion beam milling, and scanning electron microscopy at a single focal position and enables simultaneous fluorescence microscopy while milling.
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Wang, Jue [California Institute of Technology (CalTech), Pasadena, CA (United States); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)], Sica, Anthony V. [SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)], Jensen, Grant J. [Brigham Young Univ., Provo, UT (United States)], Dahlberg, Peter D. [SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Stanford Univ., CA (United States)]. 2025-10-17. Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells. https://doi.org/10.3791/68238
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