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DOE OSTI · 3005977

Develop High-Throughput Workflows for Whole-Genome Sequencing and Insertion Site Screening

Hillson, Nathan [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]·Kuo, Rita [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]·Leonard, Jack [seqWell, Inc., Beverly, MA (United States)]
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Abstract

The engineering of microbes for biomanufacturing (e.g. of fuels, chemicals, materials) applications has advanced to a stage where researchers screen genetic libraries with millions of variations each for those with enhanced productivity. This screening, however, can be slow and expensive, as screening individual variants in a high-throughput yet cost-effective manner is challenging. In this project, we aimed to reduce by 3-fold costs associated with the sequencing aspects of the screening process (to determine which genetic variant is responsible for an observed change in productivity), while being able to process over 1,000 samples per batch.

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60 APPLIED LIFE SCIENCES97 MATHEMATICS AND COMPUTING

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BibTeXRIS

Hillson, Nathan [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Kuo, Rita [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Leonard, Jack [seqWell, Inc., Beverly, MA (United States)]. 2025-10-28. Develop High-Throughput Workflows for Whole-Genome Sequencing and Insertion Site Screening. https://doi.org/10.2172/3005977

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