DOE OSTI · 3007775
Moisture Metaphenome Incubation Analysis Results
Abstract
The Birch effect, a pulse of CO2 release that occurs when dry soil is rewet, is commonly observed, yet the underlying biogeochemistry remains elusive. Using multi-omics data, real-time mass spectrometry and modeling approaches, we investigated the molecular response to rewetting of a soil microbiome exposed to drought for one and two weeks. The microbiome response was evaluated through analysis of transcript, protein, metabolite, and respiration profiles and metabolic modeling using an enhanced version of the Metabolite Expression Metabolic Network Integration for Pathway Identification and Selection (MEMPIS) algorithm (Roy Chowdhury et al, mSystems, 2019).
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Lipton, Mary S [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000177490077), Nelson, William C [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000218733929), McCullough, Hugh C [Univ. of Nebraska, Lincoln, NE (United States)] (ORCID:0009000968637474), Smith, Montana L [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000286830050), Bell, Sheryl L [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000184451375), Bramer, Lisa M [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000257557200), Hyun-Seob, Song [Univ. of Nebraska, Lincoln, NE (United States)] (ORCID:0000000221546358), Hofmockel, Kirsten S [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000315862167). 2025-12-16. Moisture Metaphenome Incubation Analysis Results. https://doi.org/10.25584/3007775
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