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DOE OSTI · 3011779

Rapid T cell engineering to counter emerging threats (Full Technical Report)

Robertson, Claire [Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)]
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Abstract

Instead of genetic engineering, we sought to determine if a rapid method of membrane protein delivery could generate functional CAR-T cells in a rapid, safer, cost-effective manner. Using cell free protein synthesis, we generated CAR proteins embedded in a nanodisc, and thus effectively solubilized the protein. We demonstrated the first functional CAR proteins outside of a cellular context. We next tested uptake into immune cells and found extremely high uptake into T cells and multiple other populations of immune cells. We found some evidence of cytotoxicity in vitro conferred by the nanodisc delivered protein.

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59 BASIC BIOLOGICAL SCIENCESBiological and medical sciences

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Robertson, Claire [Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)]. 2026-01-06. Rapid T cell engineering to counter emerging threats (Full Technical Report). https://doi.org/10.2172/3011779

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