Data for Efficient Delivery of a DNA Aptamer-Based Biosensor into Plant Cells for Glucose Sensing through Thiol-Mediated Uptake

Mou, Quanbing; Xue, Xueyi; Ma, Yuan; Banik, Mandira; Garcia, Valeria; Guo, Weijie; Wang, Jiang; Song, Tingjie; Chen, Li-Qing; Lu, Yi

doi:10.13012/b2idb-6284123_v1

DOE OSTI · 3014366

Data for Efficient Delivery of a DNA Aptamer-Based Biosensor into Plant Cells for Glucose Sensing through Thiol-Mediated Uptake

Mou, Quanbing·Xue, Xueyi·Ma, Yuan·Banik, Mandira·Garcia, Valeria·Guo, Weijie·Wang, Jiang·Song, Tingjie·Chen, Li-Qing·Lu, Yi

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Abstract

DNA aptamers have been widely used as biosensors for detecting a variety of targets. Despite decades of success, they have not been applied to monitor any targets in plants, even though plants are a major platform for providing oxygen, food, and sustainable products ranging from energy fuels to chemicals, and high-value products such as pharmaceuticals. A major barrier to progress is a lack of efficient methods to deliver DNA into plant cells. We herein report a thiol-mediated uptake method that more efficiently delivers DNA into Arabidopsis and tobacco leaf cells than another state-of-the-art method, DNA nanostructures. Such a method allowed efficient delivery of a glucose DNA aptamer sensor into Arabidopsis for sensing glucose. This demonstration opens a new avenue to apply DNA aptamer sensors for functional studies of various targets, including metabolites, plant hormones, metal ions, and proteins in plants for a better understanding of the biodistribution and regulation of these species and their functions.

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Mou, Quanbing, Xue, Xueyi, Ma, Yuan, Banik, Mandira, Garcia, Valeria, Guo, Weijie, Wang, Jiang, Song, Tingjie, Chen, Li-Qing, Lu, Yi. 2022-06-29. Data for Efficient Delivery of a DNA Aptamer-Based Biosensor into Plant Cells for Glucose Sensing through Thiol-Mediated Uptake. https://doi.org/10.13012/b2idb-6284123_v1

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