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DOE OSTI · 3018255

TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate

Beckham, Gregg [National Laboratory of the Rockies (NLR), Golden, CO (United States)] (ORCID:000000023480212X)·Reed, Michelle [National Laboratory of the Rockies (NLR), Golden, CO (United States)]·DesVeaux, Jason [National Laboratory of the Rockies (NLR), Golden, CO (United States)] (ORCID:0000000179266327)·Norton-Baker, Brenna [National Laboratory of the Rockies (NLR), Golden, CO (United States)]·Murphy, Natasha [National Laboratory of the Rockies (NLR), Golden, CO (United States)]
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Abstract

The primary objective of this project was to develop a new polyethylene terephthalate (PET) hydrolase enzyme to depolymerize relevant PET substrates for Birch Biosciences, using high-throughput protein expression, purification, and assaying systems and machine learning-guided enzyme design. As a secondary project objective, we also aimed to develop a more energy-efficient ethylene glycol (EG) recovery strategy relative to distillation.

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09 BIOMASS FUELScrystalline PETEnzymesPET hydrolase

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Beckham, Gregg [National Laboratory of the Rockies (NLR), Golden, CO (United States)] (ORCID:000000023480212X), Reed, Michelle [National Laboratory of the Rockies (NLR), Golden, CO (United States)], DesVeaux, Jason [National Laboratory of the Rockies (NLR), Golden, CO (United States)] (ORCID:0000000179266327), Norton-Baker, Brenna [National Laboratory of the Rockies (NLR), Golden, CO (United States)], Murphy, Natasha [National Laboratory of the Rockies (NLR), Golden, CO (United States)]. 2026-02-09. TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate. https://doi.org/10.2172/3018255

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