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DOE OSTI · 3022642

Integrative SP3 Workflow for Multi-PTM Proteomics Profiling (TZ-DP0)

Zhang, Tong [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]·Gluth, Austin [Washington State Univ., Pullman, WA (United States)]·Li, Xiaolu [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]·Waters, Katrina M [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000346965396)
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Abstract

The goal of the experiment was to demonstrate that the optimized multiplexed multi-PTM profiling workflow can comprehensively and quantitatively capture dynamic changes in protein abundance, cysteine oxidation, phosphorylation, and acetylation in cytokine-induced inflammatory stress in mouse pancreatic ß-cells. Global proteomic, redox proteomic, phosphoproteomic, and acetylomic were data collected from mouse Beta-TC-6 pancreatic Beta-cells, untreated (mock) and cytokine-treated Beta-cells at 4, 8, and 24 hours with 4 biological replicates. Samples were digested with trypsin and Lys-C, then analyzed by LC-MS/MS. Data were searched with MS-GF+, MASIC, and MaxQuant using PNNL's DMS processing pipeline.

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59 BASIC BIOLOGICAL SCIENCEShuman healthpredictive phenomicsproteomicsstructural biology

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Zhang, Tong [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Gluth, Austin [Washington State Univ., Pullman, WA (United States)], Li, Xiaolu [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Waters, Katrina M [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000346965396). 2026-03-09. Integrative SP3 Workflow for Multi-PTM Proteomics Profiling (TZ-DP0). https://doi.org/10.25584/3022642

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